Can anyone help with Microbiology questions?

1.)An investigator attempting to replicate Pasteur’s swan-necked flask experiment has boiled a hay infusion for 1 hour. However, she observes growth in the flask after 1 day (even when undisturbed). What is the likely explanation for this result?

~the flask was on its side? maybe?

2.)Name at least three limitations of Koch’s postulates that might limit their ability to identify the causative agent of a microbial disease.

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