You have digested your plasmids with BamHI or AlwNI. Why did you digest the plasmid with BamHI? What is your expectation if the plasmid is a reclosed vector without an insert? What is your expectation if your plasmid has an insert? Why did you digest the plasmid with AlwNI? What is your expectation if the plasmid is a reclosed vector without an insert? What is your expectation if your plasmid has an insert? On a gel, how can i tell its orientation? if there is an insert or not? What should I expect from BamH1 fragments and AlwN1 fragments on a gel?
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